Biosynthesis of Acyl Dihydroxyacetone Phosphate in Guinea Pig Liver Mitochondria

The subcellular distribution of acyl CoA: dihydroxyacetone phosphate acyl transferase in guinea pig liver.

Summarv: Upon differential centrifugation, the enzyme acyl CoA:dihydroxyacetone phosphate acyl transferase (EC 2.3.1.42) in guinea pig liver is shown to sediment in a lysosomal-peroxisomal fraction. Comparison of the distribution of the marker enzymes and of DHAP acyl transferase indicates that the acyl transferase is localized in peroxisomes (microbodies). Acyl dihydroxyacetone phosphate (acyl...

Purification and properties of acyl/alkyl dihydroxyacetone-phosphate reductase from guinea pig liver peroxisomes.

The peroxisomal acyl/alkyl dihydroxyacetone-phosphate reductase (EC 1.1.1.101) was solubilized and purified 5500-fold from guinea pig liver. The enzyme could be solubilized by detergents only at high ionic strengths in presence of the cosubstrate NADPH. Peroxisomes, isolated from liver by a Nycodenz step density gradient centrifugation, were first treated with 0.2% Triton X-100 to remove the so...

Properties of guinea pig liver peroxisomal dihydroxyacetone phosphate acyltransferase.

Glycerolipid biosynthesis in peroxisomes via the acyl dihydroxyacetone phosphate pathway.

In recent years, acyl dihydroxyacetone phosphate [acyl-DHAPt) has been shown to be a precursor of glycerolipids and glycerol-ether lipids.'.' Acyl-DHAP was discovered as a rapidly labeled lipid that was formed in crude mitochondrial fraction from 3'Pi or y-32P[ATP].3 This rapid labeling was due to the enzymatic dephosphorylation and rephosphorylation of endogenous acyl-DHAP present in the crude...

Biosynthesis of acyl dihydroxyacetone phosphate in subcellular fractions of rat liver.

The activity of acyl-CoA: dihydroxyacetone phosphate acyltransferase in rat liver was studied by measuring the formation of labeled lipid from dihydroxyacetone [W]phosphate and either palmitoyl-CoA or a mixture of palmitate, CoA, and ATP. Bovine serum albumin stimulated the activity of the enzyme several-fold. In the presence of albumin the acyltransferase activity in mitochondria was much high...